Fig. 1
From: Upregulation of brain-derived neurotrophic factor by Shiikuwasha (Citrus depressa Hayata)
Effects of Shiikuwasha extracts on brain-derived neurotrophic factor (BDNF) level in ACHN cell culture medium. ACHN cells were cultured for 24 h with the methanol extracts of Shiikuwasha peel, pulp, and seeds at 50 and 100 µg/mL. BDNF level was measured using the enzyme-linked immunosorbent assay. Each value represents BDNF level in the culture medium. ACHN cells treated with Chinpi were used as the positive control. Data are expressed as mean ± SD (controls; n = 4, other groups; n = 3). Dunnett's test; *P < 0.05 vs. control (0 µg/mL)