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Table 1 Optimised mass spectrometry parameters of tacrolimus, cyclosporin A, and ISs in CLAM-LC-MS/MS

From: Validation of an automated sample preparation module directly connected to LC-MS/MS (CLAM-LC-MS/MS system) and comparison with conventional immunoassays for quantitation of tacrolimus and cyclosporin A in a clinical setting

 

Precursor ion

   

Product ion

     

Compounds

  

Quantitation

   

Reference

  

Retention time (min)

 

m/z

 

Q1

Collision

Q3

 

Q1

Collision

Q3

 
  

m/z

pre-bias

energy

pre-bias

m/z

pre-bias

energy

pre-bias

 
   

(V)

(V)

(V)

 

(V)

(V)

(V)

 

Tacrolimus

821.5

768.3

-30

-22

-22

576.2

-30

-22

-30

0.735

[13 C,2H4]-Tacrolimus

826.5

773.4

-30

-22

-22

581.4

-30

-22

-30

0.735

Cyclosporin A

1219.8

1202.9

-36

-19

-32

1184.6

-38

-35

-36

0.783

[2H12]-Cyclosporin A

1231.7

1214.9

-36

-19

-32

1196.8

-38

-35

-36

0.780

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Journal of Pharmaceutical Health Care and Sciences

ISSN: 2055-0294

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