Table 2 Results of the validation study of QCs (tacrolimus and cyclosporin A) in CLAM-LC-MS/MS
Compounds | Calibration curve Dynamic range (ng/mL) | r2 | LLOQ (ng/mL) | Intra-assay precision (%) (%RSD) (n = 10) | Inter-assay precision (%) (%RSD) (10 days) | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
C1 | C2 | C3 | C4 | C1 | C2 | C3 | C4 | ||||
Tacrolimus | 2.0-35.6 | 0.999 | 2.0 | 97.3 (6.5) | 100.4 (3.5) | 100.4 (2.6) | 105.5 (3.6) | 94.8 (3.1) | 99.8 (4.7) | 101.7 (4.4) | 105.9 (5.1) |
Cyclosporin A | 24.3-1838.6 | 0.999 | 24.3 | 95.3 (2.5) | 97.7 (1.6) | 97.7 (1.9) | 104.3 (1.6) | 98.8 (4.0) | 99.0 (3.7) | 96.6 (3.6) | 98.7 (4.5) |