Skip to main content

Table 1 16S rRNA gene sequencing analysis and culture method results for the environmental survey of the northern ward

From: Comparison of 16S ribosomal RNA gene sequence analysis and conventional culture in the environmental survey of a hospital

The northern ward

Samples

16S rRNA analysis

Sequence length1)

%, Identity2)

Culture method

Nurses’ station

System of urinal collection

Corynebacterium sp.

Propionibacterium sp.

Neisseria sp.

Staphylococcus sp.

Actinomyces sp.

880

810

786

818

801

100

99

99

100

99

(−)

Personal computer

Acinetobacter sp.

Staphylococcus sp.

Corynebacterium sp.

Propionibacterium sp.

810

797

801

794

99

99

99

100

(−)

Faucet

Acinetobacter sp.

Serratia sp.

Staphylococcus sp.

884

790

806

99

99

99

(−)

Outlet

Acinetobacter sp.

Stenotrophomonas sp.

816

791

99

99

Enterobacter cloacae

Stenotrophomonas maltophilia

Patient’s room 1

Fence of the bed

Staphylococcus sp.

Propionibacterium sp.

Corynebacterium sp.

884

802

817

99

99

99

CNS

Aspirator

Acinetobacter sp.

Corynebacterium sp.

Staphylococcus sp.

Neisseria sp.

Bacteroides sp.

Prevotella sp.

Actinomyces sp.

802

794

793

798

819

800

786

99

99

99

100

99

99

99

(−)

Trash box

Staphylococcus sp.

Stenotrophomonas sp.

Corynebacterium sp.

805

789

814

99

99

99

CNS

Klebsiella pneumoniae

Asperugirus sp.

Patient’s room 2

Fence of the bed

Acinetobacter sp.

Corynebacterium sp.

Klebsiella sp.

Enterocococcus sp.

Fusobacterium sp.

Lactobacillus sp.

Prevotella sp.

823

820

746

738

805

812

794

99

99

99

99

99

99

99

(−)

Intubation tube

Acinetobacter sp.

Corynebacterium sp.

Staphylococcus sp.

Stenotrophomonas sp.

Fusobacterium sp.

Lactobacillus sp.

805

882

797

806

802

802

99

99

99

99

99

99

Acinetobacter baumannii

Patient’s room 3

Fence of the bed

Staphylococcus sp.

Corynebacterium sp.

Actinomyces sp.

844

889

820

99

99

99

CNS

Intubation tube

Acinetobacter sp.

Staphylococcus sp.

Stenotrophomonas sp.

804

836

801

99

99

99

Acinetobacter lwoffii

CNS

Patient’s room 4

Outlet

Stenotrophomonas sp.

803

99

Aeromonas hydrophia

Stenotrophomonas maltophilia

Curtain

Staphylococcus sp.

Streptococcus sp.

Neisseria sp.

Corynebacterium sp.

Escherichia sp.

Fusobacterium sp.

773

771

801

798

803

800

99

99

99

99

99

99

(−)

Dirty utility room

Urinal surface

Escherichia sp.

823

99

(−)

  1. CNS coagulase-negative staphylococci
  2. 1)Length of sequence used in the Basic Local Alignment Search Tool (BLAST) search
  3. 2)Based on BLAST alignment