Fig. 1

Activity levels of CYP1A2, CYP2C9, CYP2C19, CYP2D6, and CYP3A4 in Ad-P450 cells and human hepatocytes. Five human P450s were expressed in HepG2 cells as described in section titled ‘Materials and methods’. The cells (Ad-P450 cells) were cultured for 72 h and then incubated in culture medium containing P450 substrate cocktail for 5 h. Human cryopreserved primary hepatocytes seeded in type I collagen-coated 48-well plate at a density of 8.5 × 10⁴ cells/well were incubated with culture medium containing P450 substrate cocktail for 24 h. These media were collected and metabolites were analyzed by LC-MS/MS. The activity levels are shown as means ± SD (n = 3)