Table 1 MS/MS selected reaction monitoring transitions and collision energies for ions of metabolite and internal standard in the cocktail assays
From: Effect of health foods on cytochrome P450-mediated drug metabolism
| CYP1A2 | CYP2C9 | CYP2C19 | CYP2D6 | CYP3A4 | IS | |
|---|---|---|---|---|---|---|
| Substrate | Phenacetin | Diclofenac | Omeprazole | Dextromethorphan | Midazolam | Nitrazepam |
| Metabolite | Acetaminophen | 4′-Hydroxy-diclofenac | 5-Hydroxy-omeprazole | Dextrorphan | 1′-Hydroxy-midazolam | - |
| Collision energy (eV) | 19 | 33 | 13 | 42 | 23 | 25 |
| Product ion (m/z) | 110.05 | 229.95 | 213.95 | 157.05 | 324.05 | 236.10 |