Table 2 Kinetic parameters of P450-mediated drug metabolism and IC50 values of P450 inhibitors in Ad-P450 cells
From: Effect of health foods on cytochrome P450-mediated drug metabolism
Enzyme | Metabolic reaction | Inhibitor | Km (μM) | Vmax (pmol/well/hr) | IC50 (μM) |
|---|---|---|---|---|---|
CYP1A2 | Phenacetin O-deethylation | Furafylline | 16.32 | 100.30 | 0.57 |
CYP2C9 | Diclofenac 4′-hydroxylation | Sulfaphenazole | 5.87 | 239.9 | 0.53 |
CYP2C19 | Omeprazole 5-hydroxylation | Ticlopidine | 2.75 | 4.66 | 1.11 |
CYP2D6 | Dextromethorphan O-demethylation | Quinidine | 17.14 | 1.10 | 0.14 |
CYP3A4 | Midazolam 1′-hydroxylation | Ketoconazole | 1.00 | 12.30 | 0.12 |