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Table 2 Kinetic parameters of P450-mediated drug metabolism and IC50 values of P450 inhibitors in Ad-P450 cells

From: Effect of health foods on cytochrome P450-mediated drug metabolism

Enzyme Metabolic reaction Inhibitor Km (μM) Vmax (pmol/well/hr) IC50 (μM)
CYP1A2 Phenacetin O-deethylation Furafylline 16.32 100.30 0.57
CYP2C9 Diclofenac 4′-hydroxylation Sulfaphenazole 5.87 239.9 0.53
CYP2C19 Omeprazole 5-hydroxylation Ticlopidine 2.75 4.66 1.11
CYP2D6 Dextromethorphan O-demethylation Quinidine 17.14 1.10 0.14
CYP3A4 Midazolam 1′-hydroxylation Ketoconazole 1.00 12.30 0.12