Fig. 3

NGF with TJ24 or TJ68 alters both the ERK1/2 and Akt pathways. We analyzed neurite outgrowth after treatment with NGF alone, NGF and TJ24, or NGF and TJ68. Neurite outgrowth was also examined when the Erk1/2 inhibitor U0126, Akt inhibitor LY294002, or both were added to each previous condition for 72 h. Incubation with TJ24 or TJ68 promoted neurite outgrowth significantly compared to NGF alone (a, b). 0126 or LY294002 alone inhibited the promotion of neurite outgrowth by TJ24 or TJ68 partially. When both inhibitors were administered together, neurite outgrowth was significantly suppressed even following the addition of TJ24 or TJ68 (c). Results (mean ± S.E.) are representative of three independent experiments (n = 3). *p < 0.05 vs NGF alone, ^† p < 0.05 vs U0126 or LY294002, NGF: nerve growth factor; Erk1/2: extracellular signal-regulated kinase 1/2; SE: standard error