Rapid, simple, and clinically applicable high-performance liquid chromatography method for clinical determination of plasma colistin concentrations

Table 1 Summary of the method validation results

Parameter	Result
Concentration range (μg/mL)	0.10–8.0
Regression equation^a	y = 0.6496× − 0.0141
SE of slope^b	0.0230
SE of intercept^b	0.0019
Determination coefficient (r²)	0.9999
Intra-day precision (%)^c	2.69
Inter-day precision (%)^c	4.01
Intra-day accuracy (%)^c	103.9
Inter-day accuracy (%)^c	105.2
Lower limit of quantification (μg/mL)	0.10
Limit of detection (μg/mL)	0.025
Stability	acceptable
Robustness	acceptable

^ay = ax + b, where y is the ratio of the summed peak areas of colistin A and B to that of the internal standard, and x is colistin concentration (μg/mL)
^bSE, standard error (n = 3)
^cMean of four concentrations (0.10, 0.50, 2.0, and 4.0 μg/mL)

ISSN: 2055-0294