Skip to main content
Fig. 6 | Journal of Pharmaceutical Health Care and Sciences

Fig. 6

From: Low concentrations of clarithromycin upregulate cellular antioxidant enzymes and phosphorylation of extracellular signal-regulated kinase in human small airway epithelial cells

Fig. 6

Effects of CAM pretreatment on HO-1 activation (a) and mRNA expression (b) in H2O2-stimulated SAECs. In panel a, HO-1 protein levels were detected by Western blotting (upper, representative blot images; lower, quantification of bands). In panel b, HO-1 mRNA expression was measured using real-time RT-PCR. Samples were obtained from cell pellets (a) or cDNA (b) of control cells, of cells stimulated with 100 μM H2O2 alone, or of cells pretreated with 1, 5 or 10 μM CAM for 72 h before stimulation with 100 μM H2O2 for 0.5 or 1 h, respectively. The data in panel a are expressed as the HO-1/β-actin ratio. Data are presented as means ± SD of three independent experiments. **p < 0.01 vs. control cells, #p < 0.05 vs. cells stimulated with H2O2 alone, ††p < 0.01 vs. cells pretreated with 10 μM CAM

Back to article page