Table 2 LC-MS/MS assay analytical parameters of ITZ and its metabolites in human plasma
Theoretical value (ng/mL) | Intra-assay | Inter-assay | |||||
|---|---|---|---|---|---|---|---|
mean ± SD (ng/mL) | Accuracy (%) | RSD (%) | mean ± SD (ng/mL) | Accuracy (%) | RSD (%) | ||
ITZ | LLOQ (15) | 15.0 ± 0.1 | 99.8 | 0.8 | 14.1 ± 0.6 | 94.1 | 4.0 |
L (45) | 45.4 ± 0.8 | 100.8 | 1.8 | 45.4 ± 1.1 | 100.9 | 2.4 | |
M (300) | 297 ± 2.5 | 99.1 | 0.8 | 305 ± 4.6 | 101.8 | 1.5 | |
H (1200) | 1201 ± 16 | 100.1 | 1.4 | 1213 ± 26 | 101.1 | 2.1 | |
OH-ITZ | LLOQ (15) | 14.6 ± 0.5 | 97.6 | 3.3 | 14.7 ± 1.0 | 98.3 | 6.5 |
L (45) | 45.0 ± 1.3 | 100.1 | 3.0 | 45.8 ± 0.6 | 101.7 | 1.3 | |
M (300) | 299 ± 1.8 | 99.8 | 0.6 | 303 ± 7.4 | 101.1 | 2.4 | |
H (1200) | 1197 ± 21 | 99.7 | 1.8 | 1214 ± 34 | 101.1 | 2.8 | |
KT-ITZ | LLOQ (1) | 1.01 ± 0.05 | 100.9 | 5.4 | 1.03 ± 0.11 | 102.7 | 10.3 |
L (3) | 3.00 ± 0.05 | 100.0 | 1.8 | 3.02 ± 0.11 | 100.6 | 3.7 | |
M (20) | 19.9 ± 0.4 | 99.5 | 2.0 | 19.8 ± 1.0 | 99.1 | 4.9 | |
H (80) | 80.2 ± 4.0 | 100.2 | 4.9 | 81.1 ± 2.1 | 101.4 | 2.7 | |
ND-ITZ | LLOQ (1) | 1.07 ± 0.04 | 106.7 | 4.0 | 1.04 ± 0.08 | 103.5 | 8.2 |
L (3) | 2.99 ± 0.11 | 99.6 | 3.8 | 2.97 ± 0.06 | 98.8 | 2.2 | |
M (20) | 20.2 ± 1.6 | 99.9 | 8.1 | 20.5 ± 0.8 | 102.6 | 3.8 | |
H (80) | 80.0 ± 3.2 | 100.0 | 3.9 | 82.2 ± 4.4 | 102.7 | 5.3 | |