Table 1 Accuracy and precision study on the (±)-tramadol assay
From: Photostability studies on (±)-tramadol in a liquid formulation
Nominal | Run-to-run assay | Day-to-day assay | |||||
|---|---|---|---|---|---|---|---|
concentration (ng/μL) | Detected (ng/μL) | Accuracy (%) | CV (%) | Detected (ng/μL) | Accuracy (%) | CV (%) | |
(+)-tramadol | 5.0 | 5.2 ± 0.1 | 103.4 | 1.91 | 5.2 ± 0.1 | 104.3 | 1.90 |
10.0 | 9.5 ± 0.3 | 96.0 | 3.29 | 9.6 ± 0.3 | 94.9 | 3.16 | |
25.0 | 26.7 ± 0.4 | 103.9 | 1.56 | 26.0 ± 0.6 | 104.0 | 2.89 | |
50.0 | 50.4 ± 0.9 | 100.0 | 1.70 | 50.0 ± 0.8 | 100.8 | 1.41 | |
100.0 | 99.5 ± 2.2 | 100.3 | 2.20 | 100.3 ± 2.8 | 99.5 | 2.80 | |
(-)-tramadol | 5.0 | 4.9 ± 0.2 | 94.8 | 4.10 | 4.7 ± 0.2 | 98.0 | 4.30 |
10.0 | 9.5 ± 0.3 | 96.5 | 2.79 | 9.4 ± 0.2 | 94.5 | 2.25 | |
25.0 | 26.1 ± 0.9 | 104.5 | 3.44 | 26.1 ± 0.3 | 104.4 | 1.18 | |
50.0 | 50.7 ± 0.9 | 99.1 | 1.82 | 49.6 ± 0.4 | 101.4 | 0.88 | |
100.0 | 100.6 ± 2.1 | 101.2 | 2.10 | 101.2 ± 2.8 | 100.6 | 2.44 | |