Fig. 5
From: Effect of health foods on cytochrome P450-mediated drug metabolism
Effect of curcumin on P450-mediated metabolism in Ad-P450 cells. Ad-P450 cells were incubated in culture medium containing P450 substrate cocktail and curcumin (10–300 μM) for 5 h. These media were collected and metabolites were analyzed by LC-MS/MS. The P450 activities in Ad-P450 cells cultured in P450 substrate cocktail and DMSO (0.5%) for 5 h were set to 100%. The activity levels are shown as means ± SD (n = 3). The limit of quantitation is 18.90 ng/mL for acetaminophen, 39.02 ng/mL for 4′-hydroxydiclofenac, 0.24 ng/mL for 5-hydroxyomeprazole, 0.25 ng/mL for dextrorphan, and 2.14 ng/mL for 1′-hydroxymidazolam. N.D.: not detected