Fig. 1

Paclitaxel (1 μM) induced neurite retraction without cytotoxicity in PC12 cells. We evaluated cell viability and neurite length after applying varying concentrations of paclitaxel to PC12 cells. A total of 1 μM paclitaxel preserved the PC12 cells based on MTT assay, but still caused neurite regression compared with NGF application only (a, b, c). Protein expression of the neuronal differentiation markers GAP-43 and NF-L, decreased significantly after addition of 1 μM paclitaxel (d, e). Results (mean ± S.E.) are representative of three independent experiments (n = 3). **p < 0.01, *p < 0.05. NGF: nerve growth factor; GAP-43: growth-associated protein 43; NF-L: light neurofilament; SE: standard error