Fig. 7

Effects of CAM pretreatment on p-ERK expression in SAECs stimulated with H₂O₂. p-ERK and t-ERK protein levels were detected by Western blotting (upper, representative blot images; lower, quantification of bands). Samples were obtained from cell pellets of control cells, of cells stimulated with 100 μM H₂O₂ alone, or of cells pretreated with 1, 5, or 10 μM CAM for 72 h before stimulation with 100 μM H₂O₂ for 0.5 h. The data are expressed as the p-ERK/t-ERK ratio. Data are presented as means ± SD of three independent experiments. ^*p < 0.05, ^**p < 0.01 vs. control cells, ^##p < 0.01 vs. cells stimulated with H₂O₂ alone, ^†p < 0.05 vs. cells pretreated with 10 μM CAM