Fig. 8

The effect of mixing of Otsuka glucose injection with Humulin®R in the experiments of simple mixing in the vials. Humulin®R was diluted 5-fold with Otsuka glucose injection (5%) (a) and Otsuka glucose injection (5%, pH 3.5) (b). Turbidity (●), fluorescence intensity (■), and pH (▲) were evaluated. The isoelectric point of human insulin in Humulin®R and the pH range of the original formulation of Humulin®R are expressed as a dotted line and filled area in grey. Each value is expressed as the mean ± S.D. (n = 4–10)