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Table 2 Experiments using side-by-side diffusion cells

From: Investigation of factors that cause insulin precipitation and/or amyloid formation in insulin formulations

Formulation on

the donor side

Solution on

the other side

Change in the formulation applied

n

I

Humulin®R

Otsuka normal saline

m-Cresol concentration

pH

6

II

Humulin®R

PBS (pH 7.4)

m-Cresol concentration

5

III

Humulin®R

Otsuka normal saline

+ m-cresol (2.5 mg/mL)

pH

6

IV

Humulin®R

PBS (pH 5.5)

pH, can induce isoelectric precipitation

m-Cresol concentration

3

V

Humulin®R

PBS (pH 6.5)

pH, cannot induce isoelectric precipitation

m-Cresol concentration

5