Fig. 4
Relationships of energy-dependent endocytosis pathway and skin penetration of FEL in FEL-NP gel. A Franz diffusion cell with rat skin was used in the in vitro skin penetration experiments. Penetration profiles (A) and AUCpenetration values (B) of FEL-NP gel at 4 and 37 °C. Penetration profiles (C) and AUCpenetration values (D) of FEL-NP gel treated with endocytosis inhibitors (nystatin, dynasore, rottlerin, and cytochalasin D). n = 8. * P < 0.05, vs. 4 °C group for each category. #P < 0.05, vs. vehicle for each category. The penetration of FEL-NP gel was significantly attenuated by inhibition of energy-dependent endocytosis, such as CME.